《植物生理学报》 2015, 51(10): 1706-1718

大豆AP2/ERF基因家族的分子进化分析

魏海超^1,2, 刘媛¹, 豆明珠³, 杨素欣¹, 冯献忠^1,*

¹中国科学院东北地理与农业生态研究所, 大豆分子设计育种重点实验室, 长春130102; ²中国科学院大学, 北京100049; ³山东师范大学生命科学学院, 山东省高校系统生物学重点实验室, 济南250014

通信作者：冯献忠；E-mail: fengxianzhong@iga.ac.cn；Tel: 0431-85655051

摘　要：

AP2/ERF类型基因家族在植物发育中起着重要的作用, 本文鉴定了大豆AP2/ERF基因家族, 分析了该基因家族的进化途径, 研究了其在种子发育过程中的基因表达模式, 并对其功能进行了预测。研究表明: 大豆AP2/ERF家族包括355个基因, 分属于AP2、RAV和ERF三个亚家族; AP2/ERF基因在进化过程中出现了两次较大的分歧; 发现了7个与大豆分化显著相关的AP2/ERF基因, 利用拟南芥直系同源基因对这些基因的功能进行了预测; 在大豆种子发育的过程中, 该家族基因呈现10类不同的表达模式, 在种子发育的各个过程中起着不同的作用。

关键词：大豆; AP2/ERF基因家族; 进化分析; 表达谱分析

收稿：2015-07-21   修定：2015-09-14

资助：国家自然基金面上项目(31171571)和国家自然科学基金重大研究计划培育项目(91131008)。

Molecular Evolution of AP2/ERF Gene Family in Glycine max

WEI Hai-Chao^1,2, LIU Yuan¹, DOU Ming-Zhu³,YANG Su-Xin¹, FENG Xian-Zhong^1,*

¹Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; ²University of Chinese Academy of Sciences, Beijing 100049, China; ³Key Laboratory of Systems Biology in Universities of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250014, China

Corresponding author: FENG Xian-Zhong; E-mail: fengxianzhong@iga.ac.cn; Tel: 0431-85655051

Abstract:

AP2/ERF gene family plays an important role in the development of plant. In this paper, we identified the soybean AP2/ERF gene family, analyzed its evolutionary pathway, and studied the expression pattern of its members during seed development. The results showed that the soybean AP2/ERF gene family contained 355 genes which could be classified into AP2, RAV and ERF three subfamilies, which experienced two divergence events during the soybean evolution. Seven of them were characterized associating with the differentiation between cultivated soybean and wild soybean during the domestication, and their functions were predicted by their orthologous in Arabidopsis. Ten types of expression pattern of this gene family were revealed by expression profiles during the seed development of soybean, which play various roles in the seed development.

Key words: soybean; AP2/ERF gene family; phylogenetic analysis; expression profile analysis